ABSTRACT
OBJECTIVE:To observe the antiviral effects of Qingre jiedu soft capsule(ADSC)against influenza A H1N1 virus in vivo,and to provide a experimental support for clinical therapy of influenza A H1N1 virus. METHODS:BALB/c mice were ran-domly divided into normal control group,model control group,positive drug high-dose,medium-dose and low-dose groups [oselta-mivir phosphate capsule,0.04,0.02,0.01 g/(kg·d)] and ADSC high-dose,medium-dose and low-dose groups [1.5,0.75,0.375 g/(kg·d)].Except for normal control group,others groups were given influenza A H1N1 virus with titer 1.6×10-5.2 via nasal cavity to induce poisoned mice model;6-8 h after modeling,they were given relevant medicine intragastrically,once a day,for 5 days. After medication,the change of body weight within 7 d were observed in mice;the mortality and death prevention rate within 15 d,mean survival days(MSDs)were calculated in mice.Other mice were selected and grouped,and they were given same drugs as above. 8 h after last medication,lung index and inhibition rate of lung index were determined in mice.RESULTS:In model control group,the body weight of mice decreased significantly since 5th day,and mice death was beginning to occur since 8th day(mortal-ity of 85.7% within 15 d);the lung index was increased significantly compared to normal control group (P<0.01). Both ADSC and oseltamivir phosphate capsule could slow down the decrease of body weight in mice,decreased the mortality and lung index of mice,and prolonged MSDs;the MSDs of mice in ADSC high-dose,positive drug high-dose and medium-dose groups were signifi-cantly higher than model control group(P<0.05),and lung index was significantly lower than model control group except that of ADSC low-dose group(P<0.05). CONCLUSIONS:ADSC has certain antiviral effect against influenza A H1N1 virus in vivo.
ABSTRACT
<p><b>OBJECTIVE</b>Our study aims to evaluate the antiviral effects of Ouyi antipyretic detoxicate soft capsule against influenza A virus H1N1 in vivo, so as to find an effective Chinese medicinal formulae for the treatment of the virus infection, which may lay a theoretical foundation for clinic treatment of patient infected with Influenza A Virus H1N1.</p><p><b>METHOD</b>With the observation of cytopathic effect (CPE) that induced by virus ,we investigated viral inhibition rate by MTT colorimetric assay and valued antiviral activity of drugs by therapeutic index (TI) . Meanwhile, Oseltamivir phosphate capsule (Tamiflu) was used as positive control , we carried out experiments through the three ways of preventive effect, direct inactivation and propagation inhibition.</p><p><b>RESULT</b>Ouyi antipyretic detoxicate soft capsule could effectively inhibit cytopathic effect (CPE) that induced by Influenza A Virus H1N1. The preventive effect, direct inactivation , and inhibition of endogenous multiplication of Ouyi antipyretic detoxicate soft capsule and Tamiflu against influenza A virus H1N1 were observed. And three types of action therapeutic index (TI) from Ouyi antipyretic detoxicate soft capsule were (15.5 +/- 0.71), (0.55 +/- 0.071), (6.4 +/- 1.27) severally, comparing Tamiflu with (0.4 +/- 0.14), (1.88 +/- 0.29), (4.6 +/- 0.15), respectively.</p><p><b>CONCLUSION</b>Ouyi antipyretic detoxicate soft capsule showed more remarkable preventive effect than Tamiflu in vitro (P<0.01). The possible mechanism of the antiviral activity observed in our study might be the protection of the MDCK cells from viral infection by inhibiting the viral absorption. We need a further study to certify three effects in vivo.</p>
Subject(s)
Animals , Dogs , Antipyretics , Pharmacology , Antiviral Agents , Pharmacology , Capsules , Cell Line , Influenza A Virus, H1N1 Subtype , Inhibitory Concentration 50ABSTRACT
In order to evaluate the anti-influenza virus activity of the effective monomer from Folium Isatidis (FI) in vivo, we established mice model with viral pneumonia and divided them into 3 different dose groups, then observed their lung indexes, pulmonary pathological changes, pulmonary virus hemagglitination titers, living time and death rates. The results showed that the monomer could reduce the pulmonary index from 2.64 to 1.93, 1.63 and 1.40 (P<0.01) and decrease the hemagglitination titer from 1.15 to 0.84, 0.70 and 0.59 (P<0.01). In addition,the living time from 5.1d to 6.5d, 8.4d and 8.9d respectively(P<0.01). The high dose (75 mg/kg/d) has the similar effect with 100 mg/kg/d dose of virazole(P>0.05), and more effective than 200 mg/kg/d dose of antiviral liquor (P<0.05).
ABSTRACT
Objective To observe the effect of short wave diathermy on the functioning of dendritic cells ( DCs) derived from the spleens of hepatitis B virus ( HBV) transgenic mice. Methods HBV transgenic mice were treated with short wave diathermy for 10 days. Dendritic cells were then separated from their spleens and incubated with granulocyte-macrophage colony-stimulating factor ( GM-CSF) and interleukin-4 ( IL-4 ). The phenotypes of the DCs were assayed by flow cytometry ( FCM) ,IL-12 released by the DCs and IL-10 and IFN-? produced by the T cells in a mixed lymphocyte reaction( MLR) were measured using ELISA. Results Short wave diathermy could enhance the DCs' phenotype expression, increase IL-12 levels ( P